Abstract: Objective　Microsatellite DNA markers were employed to assess the genetic quality of SPF “Shendan No.1” Coturnix populations.Method　To assess genetic diversity, 57 SPF “Shendan No.1” Coturnix individuals were genotyped at 23 microsatellite loci. Result　A total of 195 alleles were detected, averaging 8.48 alleles per locus, with GUJ0029 exhibiting the highest allelic richness (17 alleles), followed by GUJ0071(16 alleles), while UBC0004 showed minimal variation (3 alleles). Among the 23 loci, the polymorphism analysis revealed that 19 loci were highly polymorphic (PIC> 0.5), whereas UBC0005, GUJ0063, and GUJ0084 displayed moderate polymorphism, and UBC0004 was the only low-polymorphic locus. The population exhibited amean effective allele number of 4.385 5, showing a high degree of polymorphism. And anaverage heterozygosity of 0.642 3, indicating robust genetic diversity. These values, consistent with the expected range (0.5-0.7) for closed colonies, reflect the controlled breeding history of SPF coturnix.Conclusion　Our findings confirmed that the SPF “Shendan No.1” quail population retains sufficient genetic diversity while meeting the criteria for closed colony maintenance. Thus, it serves as asuitable foundation stock for standardized laboratory animal breeding programs.

Key words: SPF coturnix, genetic quality control, microsatellite markers, closed colony

摘要： 目的　利用微卫星DNA标记技术对无特定病原体(SPF)“神丹1号”鹌鹑群体进行遗传质量分析。方法 采用23对微卫星标记位点对SPF“神丹1号”鹌鹑的57个个体进行遗传质量分析。结果　SPF鹌鹑在23个微卫 星位点上共检测到195个等位基因,平均每个位点含有8.48个等位基因。其中,GUJ0029位点上等位基因数最多, 达17个,GUJ0071位点上有16个,UBC0004位点上等位基因数最少,仅3个。23个位点中,19个位点多态信息含 量大于0.5,呈现出高度多态,UBC0005、GUJ0063、GUJ0084呈现出中度多态,仅UBC0004为低度多态位点。SPF鹌 鹑群体的平均有效等位基因数为4.385 5,具有高度的多态性,观测杂合度为0.642 3,位于0.5~0.7区间,符合封 闭群的遗传特征,与SPF鹌鹑培育背景一致。结论　该SPF“神丹1号”鹌鹑群体内存在丰富的遗传多样性,符合 封闭群建立的遗传特征要求,可进一步作为实验动物标准化培育的基础群体。

关键词: SPF鹌鹑, 遗传质量控制, 微卫星标记, 封闭群